if you have properly wired up your 4AGE complete with circuit opening relay and main relays - you will have no problems in starting and running..
to achieve proper octane rating for this engine, you need to mix 40% HOBC with 60% regular.. and for the love of GOD do not use plain water in the cooling system, the 20 valve head has tiny crevices for coolant flow - using water clogs them with rust and you have hot spots which induce knock at which time the ECU retards timing very quickly. (hence everyone suggests to use HOBC) which is not a fuel in the first place its a blending component
Use genuine toyota pink coolant after fully washing the inside of the cooling system, fill it with a 50/50 mixture - the thermostat should be in place and do remember to connect up the cooling fan terminal on the ECU.. This tells the ECU if the fan is operating - if it doesnt get a signal your performance will be limited..